Publications (Citation Index)

152. Karnaouri A., Asimakopoulou G., Kalogiannis K.G., Lappas A.A., Topakas* E. (2021). Efficient production of nutraceuticals and lactic acid from lignocellulosic biomass by combining organosolv fractionation with enzymatic/fermentative routes. Bioresource Technology, 341, 125846; doi: 10.1016/j.biortech.2021.125846.


151. Dedes G., Karnaouri A., Marianou A.A., Kalogiannis K.G., Michailof C.M., Lappas A.A., Topakas* E. (2021). Conversion of organosolv pretreated hardwood biomass into 5-hydroxymethylfurfural (HMF) by combining enzymatic hydrolysis and isomerization with homogeneous catalysis. Biotechnology for Biofuels, 14:172; doi: 10.1186/s13068-021-02022-9.


150. Paz A., Chalima A., Topakas* E. (2021). Biorefinery of exhausted olive pomace through the production of polygalacturonases and omega-3 fatty acids by Crypthecodinium cohnii. Algal Research, 59, 102470; doi: 10.1016/j.algal.2021.102470.


149. Nikolaivits E. Pentari C., Kosinas C., Feiler C.G., Spiliopoulou M., Weiss M.S., Dimarogona* M., Topakas* E. (2021). Unique features of the bifunctional GH30 from Thermothelomyces thermophila revealed by structural and mutational studies. Carbohydrate Research, 273, 118553; doi: 10.1016/j.carbpol.2021.118553.


148. Zouraris D., Karnaouri A., Pandis P.K., Argirusis Chr., Topakas E., Karantonis A. (2021). On the interaction of lytic polysaccharide monoxygenases (LPMOs) with phosphoric acid-swollen cellulose (PASC). Journal of Electroanalytical Chemistry, 897, 115540; doi: 10.1016/j.jelechem.2021.115540.


147. Milovanovic J., Gunduz M.G., Zerva A., Petkovic M., Beskoski V., Thomaidis N., Topakas E., Nikodinovic-Runic J. (2021). Synthesis and laccase-mediated oxidation of new condensed 1,4-dihydropyridine derivatives. Catalysts, 11, 727; doi: 10.3390/catal11060727.


146. Stramarkou M., Oikonomopoulou V., Chalima A., Boukouvalas C., Topakas E., Krokida M. (2021). Optimization of green extractions for the recovery of docosahexaenoic acid (DHA) from Crypthecodinium cohnii. Algal Research, 58, 102374; doi: 10.1016/j.algal.2021.102374


145. Nikolaivits E., Pantelic B., Azeem M., Taxeidis G., Babu R., Topakas E., Fournet M.B., Nikodinovic-Runic J. (2021). Progressing plastics circularity: a review of mechano-biocatalytic approaches for waste plastic (re)valorization. Frontiers in Bioengineering and Biotechnology, 696040; doi: 10.3389/fbioe.2021.696040.


144. Zouraris D., Karnaouri A., Xydou R., Topakas E., Karantonis A. (2021). Exploitation of enzymes for the production of biofuels: electrochemical determination of kinetic parameters of LPMOs. Applied Sciences, 11, 4715; doi: 10.3390/app11114715


143. Chalima A., Fernandez de Castro L., Burgstaller L., Sampaio P., Carolas A.L., Gildemyn S., Velghe F., Ferreira B., Pais C., Neureiter M., Dietrich T., Topakas* E. (2021). Waste derived volatile fatty acids as carbon source for added-value fermentation approaches. FEMS Microbiology Letters, 368, fnab054; doi: 10.1093/femsle/fnab054.


142. Zerva A., Chorozian K., Kritikou A.S., Thomaidis N.S., Topakas* E. (2021). β-Glucosidase and β-galactosidase-mediated transglycosylation of steviol glucosides utilizing industrial byproducts. Frontiers in Bioengineering and Biotechnology, 9, 685099; doi: 10.3389/fbioe.2021.685099.


141. Zhang Y., Zhang Y., Song M., Topakas E., Yu Q., Yuan Z., Wang Z., Guo Y. (2021). Combining Michaelis-Menten theory and enzyme deactivation reactions for the kinetic study of enzymatic hydrolysis by different pretreated sugarcane bagasse. Process biochemistry, 105, pp. 72-78; doi: 10.1016/j.procbio.2021.03.023.


140. Topakas* E. (2021). Remembering Prof. Dr. Peter Grunwald (1941-2020). Catalyst, 11, 422; doi: 10.3390/catal11040422


139. Zerva A., Pentari C., Termentzi A., America A.H.P., Zouraris D., Bhattacharya K.S., Karantonis A., Zervakis G.I., Topakas* E. (2021). Discovery of two novel laccase-like multicopper oxidases from Pleurotus citrinopileatus and their application in phenolic oligomer synthesis. Biotechnology for Biofuels, 14:83; doi: 10.1186/s13068-021-01937-7.


138. Nikolaivits E., Valmas A., Dedes G., Topakas E., Dimarogona M. (2021). Considerations on activity determinants of fungal polyphenol oxidases based on mutational and structural studies. Applied and Environmental Microbiology, 87(11), e00396-21; doi: 10.1128/AEM.00396-21


137. Zerva A., Limnaios A., Kritikou A.S., Thomaidis N.S., Taoukis P., Topakas* E. (2021). A novel thermophile β-galactosidase from Thermothielavioides terrestris producing galactooligosaccharides from acid whey. New Biotechnology, 63, pp. 45-53; doi: 10.1016/j.nbt.2021.03.002.


136. Djapovic M., Milivojevic D., Ilic-Tomic T., Lješević M., Nikolaivits E., Topakas E., Maslak V., Nikodinovic-Runic J. (2021) Synthesis and characterization of polyethylene terephthalate (PET) precursors and potential degradation products: Toxicity study and application in discovery of novel PETases. Chemosphere, 275, 130005; doi:10.1016/j.chemosphere.2021.130005.


135. Nikolaivits E., Siaperas R., Agrafiotis A., Ouazzani J., Gioti A., Topakas E. (2021) Functional and transcriptomic investigation of laccase activity in the presence of PCB29 identifies two novel enzymes and the multicopper oxidase repertoire of a marine-derived fungus. Science of the Total Environment, 775, 145818; doi: 10.1016/j.scitotenv.2021.145818.


134. Zerva A., Tsafantakis N., Topakas* E. (2021). Evaluation of Basidiomycetes Wild Strains Grown in Agro-Industrial Residues for Their Anti-Tyrosinase and Antioxidant Potential and for the Production of Biocatalysts. Fermentation, 7, 19; doi: 10.3390/fermentation7010019.


133. Karnaouri  A., Zerva A., Christakopoulos P., Topakas* E. (2021). Screening of recombinant lignocellulolytic enzymes through rapid plate assays. Book Chapter, in “Protein Downstream processing: Design, Development and Application of High and Low-Resolution Methods, Second Edition”. Ν. Labrou (Ed), Humana Press-Springer, 2178:479-503; doi: 10.1007/978-1-0716-0775-6_30


132. Topakas* E. (2021). Top 5 cited paper in the Section of Biocatalysis. Catalyst, 11(1), 27; doi: 10.3390/catal11010027.


131. Frakolaki G., Giannou V., Topakas E., Tzia C. (2021). Effect of various encapsulating agents on the beads’ morphology and the viability of cells during BB-12 encapsulation through extrusion. Journal of Food Engineering, 294, 110423; doi: 10.1016/j.jfoodeng.2020.110423.


130. Paz A., Karnaouri A., Templis C.C., Papayannakos N., Topakas* E. (2020). Valorization of exhausted olive pomace for the production of omega-3 fatty acids by Crypthecodinium cohnii. Waste Management, 118, pp. 435-444; doi: 10.1016/j.wasman.2020.09.011.


129. Zerva A., Pentari C., Grisel S., Berrin J-G., Topakas* E. (2020). A new synergistic relationship between xylan-active LPMO and xylobiohydrolase to tackle recalcitrant xylan. Biotechnology for Biofuels, 13:142; doi: 10.1186/s13068-020-01777-x.


128. Dedes G., Karnaouri A., Topakas* E. (2020). Novel routes in transformation of lignocellulosic biomass to furan platform chemicals: from pretreatment to enzyme catalysis. Catalyst, 10, 743; doi: 10.3390/catal10070743


127. Karnaouri A., Asimakopoulou G., Kalogiannis K.G., Lappas A., Topakas* E. (2020). Efficient D-lactic acid production by Lactobacillus delbrueckii subsp. bulgaricus through conversion of organosolv pretreated lignocellulosic biomass. Biomass and Bioenergy, 140, 105672; doi:10.1016/j.biombioe.2020.105672


126. Kalogiannis K.G., Karnaouri A., Michailof C., Tzika A.M., Asimakopoulou G., Topakas E., Lappas A.A. (2020). OxiOrganosolv: a novel acid free oxidative organosolv fractionation for lignocellulose fine sugar streams. Bioresource Technology, 313, 123599; doi: 10.1016/j.biortech.2020.123599


125. Zerva A., Pentari C., Topakas* E. (2020). Cross-linked enzyme aggregates (CLEAs) of laccases from Pleurotus citrinopileatus induced in olive oil mill wastewater (OOMW). Molecules, 25,2221; doi: 10.3390/molecules25092221


124. Nikolaivits E., Agrafiotis A., Baira E., Le Goff G., Tsafantakis N., Chavanich S.A., Benayahu Y., Ouazzani J., Fokialakis N., Topakas* E. (2020). Degradation mechanism of 2,4-dichlorophenol by fungi isolated from marine invertebrates. International Journal of Molecular Sciences, 21,3317; doi:10.3390/ijms21093317


123. Nikodinovic-Runic J., Topakas* E. (2020). Special Issue on Environmental Biocatalysis. Editorial in Catalyst, 10(5), 490; doi:10.3390/catal10050490


122. Zouraris D., Zerva A., Topakas E., Karantonis A. (2020). FTacV study of electroactive immobilized enzyme/free substrate reactions: enzymatic catalysis of epinephrine by a multicopper oxidase from Thermothelomyces thermophila. Bioelectrochemistry, 13, 107538; doi: 10.1016/j.bioelechem.2020.107538


121. Gioti A., Siaperas R., Nikolaivits E., Le Goff G., Ouazzani J., Kotoulas G., Topakas E. (2020). Draft genome sequence of a Cladosporium species isolated from the mesophotic ascidian Didemnum maculosum. Microbiology Resource Announcements, 9:e00311-20; doi: 10.1128/ MRA.00311-20


120. Paz A., Nikolaivits E., Topakas* E. (2020). Valorization of olive mill wastewater towards the production of L-asparaginases. Biomass Conversion and Biorefinery, 11, pp. 539-546; doi:10.1007/s13399-020-00725-x


119. Dimarogona M., Topakas E., Christakopoulos P., Chrysina E.D. (2020). The crystal structure of a Fusarium oxysporum feruloyl esterase that belongs to the tannase family.  FEBS Letters, 594, pp. 1738-1749; doi: 10.1002/1873-3468.13776


118. Giakoumakis G., Karnaouri A., Topakas E., Sidiras D. (2020). Simulation and optimization of combined acid pretreatment and enzymatic saccharification of medical cotton waste. Biomass Conversion and Biorefinery, 11, pp. 515-526;


117. Karnaouri A., Chalima A., Kalogiannis K., Varamogianni D., Lappas A., Topakas* E. (2020). Utilization of lignocellulosic biomass towards the production of omega-3 fatty acids by the heterotrophic marine microalga Crypthecodinium cohnii. Bioresource Technology, 303, 122899; doi: 10.1016/j.biortech.2020.122899


116. Chalima A., Taxeidis G., Topakas* E. (2020). Optimization of the production of docosahexaenoic fatty acid by the heterotrophic microalga Crypthecodinium cohnii utilizing a dark fermentation effluent. Renewable Energy, 152, pp. 102-109; doi:10.1016/j.renene.2020.01.041


115. Zerva A. Simic S., Topakas* E. Nikodinovic-Runic J. (2019). Applications of microbial laccases: patent review of the past decade (2009-2019). Catalyst, 9, 1023; doi:10.3390/catal9121023


114. Nikolaivits E., Agrafiotis A., Termentzi A., Machera K., Le Goff G., Alvarez P., Chavanich S., Benayahu Y., Ouazzani J., Fokialakis N., Topakas* E. (2019). Unraveling the detoxification mechanism of 2,4-dichlorophenol by marine-derived mesophotic symbiotic fungi isolated from marine invertebrates. Marine Drugs, 17, 564; doi:10.3390/md17100564


113. Mandic M., Djokic L., Nikolaivits E., Prodanovic R., O’Connor K., Jeremic* S., Topakas* E., Nikodinovic-Runic* J. Identification and characterization of new laccase biocatalysts from Pseudomonas species suitable for degradation of synthetic textile dyes. Catalysts. 2019, 9, 629,


112. Katsimpouras C., Dedes G., Thomaidis N.S., Topakas* E. (2019). A novel fungal GH30 xylanase with xylobiohydrolase auxiliary activity. Biotechnology for Biofuels 12:120,


111. Chalima A., Hatzidaki A., Karnaouri A., Topakas* E. (2019). Integration of a dark fermentation effluent in a microalgal-based biorefinery for the production of high-added value omega-3 fatty acids. Applied Energy, 241, pp. 130-138.


110. Karnaouri A., Antonopoulou I., Zerva A., Dimarogona M., Topakas E., Rova U., Christakopoulos P. (2019). Thermophilic enzyme systems for efficient conversion of lignocellulose to valuable products: structural insights and future perspectives for esterases and oxidative catalysts. Bioresource Technology, 279, pp. 362-372.


109. Zerva A., Koutroufini E., Kostopoulou I., Detsi A., Topakas* E. (2019). A novel thermophilic laccase-like multicopper oxidase from Thermothelomyces thermophila and its application in the oxidative cyclization of 2’,3,4-trihydroxychalcone. New Biotechnology, 49, pp. 10-18.


108. Nikolaivits E., Kanelli M., Dimarogona M., Topakas* E. (2018). A middle-aged enzyme still in its prime: Recent advances in the field of cutinases. Catalysts, 8, 612; doi:10.3390/catal8120612.


107. Madhu N.M., Zouraris D., Karantonis A., Topakas E., Sandgren M., Rova U., Christakopoulos P. (2018). Effect of lignin fractions isolated from different biomass sources on cellulose oxidation by fungal Lytic Polysaccharide Monoxygenases. Biotechnology for Biofuels, 11:296;


106. Nikolaivits E., Dimarogona M., Karagiannaki I., Chalima A., Fishman A., Topakas* E. (2018). Characterization and protein engineering of a novel versatile fungal polyphenol oxidase with chlorophenol bioremediation potential. Applied and Environmental Microbiology, 84(23), e01628-18.


105. Katsimpouras C., Dedes G., Bistis P., Kekos D., Kalogiannis K., Topakas* E. (2018). Acetone/water oxidation of corn stover for the production of bioethanol and prebiotic oligosaccharides. Bioresource Technology, 270, pp. 208-215.


104. Zouraris D., Dimarogona M., Karnaouri A., Topakas E., Karantonis A. (2018).  Direct electron transfer of LPMOs and determination of their formal potentials by large amplitude fourier transform alternating current cyclic voltammetry. Bioelectrochemistry, 124, pp. 149-155.


103. Kanelli M., Mandic M., Kalakona M., Vasilakos S., Kekos D., Nikodinovic-Runic J., Topakas* E. (2018). Microbial production of violacein and process optimization for dyeing polyamide fabrics with acquired antimicrobial properties. Frontiers in Microbiology, 9:1495; doi: 10.3389/fmicb.2018.01495


102. Papaspyridi L.-M., Zerva A., Topakas* E. (2018). Biocatalytic synthesis of fungal β-glucans. Catalysts, 8, 274; doi:10.3390/catal8070274


101. Karnaouri A., Topakas E., Matsakas L., Rova U., Christakopoulos P. (2018). Fine-tuned enzymatic hydrolysis of organosolv pretreated forest matereials for the efficient production of cellobiose. Frontiers in Chemistry, doi: 10.3389/fchem.2018.00128


100. Salapa I., Topakas E., Sidiras D. (2018). Simulation and optimization of barley straw organosolv pretreatment. Industrial Crops & Products, 113, pp. 80-88.


99. Valta K., Papadaskalopoulou C., Dimarogona M., Topakas E. (2019). Bioethanol from waste – prospects and challenges of current and emerging technologies. Book Chapter 18 in “Byproducts from Agriculture and Fisheries: Adding Value for Food, Feed, Pharma and Fuels”. Simpson B.K., Aryee A.N., Toldra F., Wiley, ISBN: 978-1-119-38397, pp. 421-456.


98. Chalima A., Oliver L., de Castro L.F., Karnaouri A., Dietrich T., Topakas* E. (2017). Utilization of volatile fatty acids from microalgae for the production of high added value compounds. Fermentation, 3, 54; doi:10.3390/fermentation3040054.


97. Frakolaki G., Giannou V., Topakas E., Tzia C. (2018). Chemical characterization and breadmaking potential of spelt versus wheat flour. Journal of Cereal Science,79, pp. 50-56.


96. Katsimpouras C., Zacharopoulou M., Matsakas L., Rova U., Christakopoulos P., Topakas* E. (2017). Sequential high gravity ethanol fermentation and anaerobic digestion of steam explosion and organosolv pretreated corn stover. Bioresource Technology, 244, pp. 1129-1136.


95. Zouraris D., Zerva A., Topakas E., Karantonis A. (2017). Kinetic and amperometric study of the MtPerII peroxidase isolated from the ascomycete fungus Myceliophthora thermophila. Bioelectrochemistry, 118, pp. 19-24.


94. Nikolaivits E., Termentzi A., Skaltsounis A-L., Fokialakis N., Topakas* E. (2017). Enzymatic tailoring of oleuropein from Olea europaea leaves and product identification by HRMS/MS spectrometry. Journal of Biotechnology, 253, pp.48-54.


93. Karnaouri A., Muraleedharan M.N., Dimarogona M., Topakas E., Sandgren M., Christakopoulos P. (2017). Recombinant expression of thermostable processive MtEG5 endoglucanase and its synergism with MtLPMO from Myceliophthora thermophila during the hydrolysis of lignocellulosic substrates. Biotechnology for Biofuels, 10:126; DOI 10.1186/s13068-017-0813-1.


92. Nikolaivits E., Makris G., Topakas* E. (2017). Immobilization of a cutinase from Fusarium oxysporum and application in pineapple flavor synthesis. Journal of Agricultural and Food Chemistry, 65(17), pp. 3505-3511.


91. Salapa I., Katsimpouras C., Topakas E., Sidiras D. (2017). Organosolv pretreatment of wheat straw for efficient ethanol production using various solvents. Biomass and Bioenergy, 100, pp. 10-16.


90. Katsimpouras C., Kalogiannis K.G., Kalogianni A., Lappas A.A., Topakas* E. (2017). Production of high concentrated cellulosic ethanol by acetone/water oxidized pretreated beech wood. Biotechnology for Biofuels, 10:54; DOI 10.1186/s13068-017-0737-9


89. Nikolaivits E., Dimarogona M., Fokialakis N., Topakas* E. (2017). Marine-derived biocatalysts: importance, accessing and application in aromatic pollutant bioremediation. Frontiers in Microbiology, 8, Article 265; doi: 10.3389/fmicb.2017.00265.


88. Zerva A., Papaspyridi L-M., Christakopoulos P., Topakas* E. (2017). Valorization of olive mill wastewater for the production of β-glucans from selected basidiomycetes. Waste and Biomass Valorization, 8, pp. 1721-1731; DOI 10.1007/s12649-017-9839-7.


87. Kanelli M., Topakas* E. (2017). Acylation of soluble polysaccharides in a biphasic system catalyzed by a CE2 acetyl esterase. Carbohydrate Polymers, 163, pp. 208-215.


86. El-Gamal R, Nikolaivits E., Zervakis G.I., Abdel-Maksoud G., Topakas E., Christakopoulos P. (2016). The use of chitosan in protecting wooden artifacts from damage by mold fungi. Electronic Journal of Biotechnology, 24, pp. 70-78;


85. Schär A., Sprecher I., Topakas E., Faulds C.B., Nystroem L. (2016). Hydrolysis of nonpolar n-alkyl ferulates by feruloyl esterases. Journal of Agricultural and Food Chemistry, 64, pp. 8549-8554; DOI: 10.1021/acs.jafc.6b02694.


84. Kanelli M., Vasilakos S., Ladas S., Symianakis E., Christakopoulos P., Topakas* E. (2016). Surface modification of pοlyamide 6.6 fibers by enzymatic hydrolysis. Process Biochemistry, in Press.


83. Nikolaivits E., Kokkinou A., Karpusas M., Topakas* E. (2016). Microbial host selection and periplasmic folding in Escherichia coli affect the biochemical characteristics of a cutinase from Fusarium oxysporum. Protein Expression and Purification, 127, pp. 1-7; doi: 10.1016/j.pep.2016.06.002.


82. Antonopoulou I., Varriale S., Topakas E., Rova U., Christakopoulos P., Faraco V. (2016). Enzymatic synthesis of bioactive compounds with high potential for cosmeceutical application. Applied Microbiology and Biotechnology, 100(15), pp. 6519-6543; doi: 10.1007/s00253-016-7647-9.


81. Katsimpouras C., Dimarogona M., Petropoulos P., Christakopoulos P., Topakas* E. (2016). A thermostable GH26 endo-β-mannanase from Myceliophthora thermophila capable of enhancing lignocellulose degradation. Applied Microbiology and Biotechnology, 100(19), pp. 8385-8397; doi: 10.1007/s00253-016-7609-2.


80. Zerva A., Manos N., Vouyiouka S., Christakopoulos P., Topakas* E. (2016). Bioconversion of biomass-derived phenols catalyzed by Myceliophthora thermophila laccase. Molecules, 21(5), 550; doi:10.3390/molecules21050550.


79. Katsimpouras C., Christakopoulos P., Topakas* E. (2016). Acetic acid-catalyzed hydrothermal pretreatment of corn stover for the production of bioethanol at high-solids content. Bioprocess and Biosystems Engineering, 39(9), pp. 1415-1423; doi:10.1007/s00449-016-1618-5.


78. Nikolaivits E., Norra G-F, Voutsas E., Topakas* E. (2016). Cutinase from Fusarium oxysporum catalyzes the acylation of tyrosol in an aqueous medium: optimization and thermodynamic study of the reaction. Journal of Molecular Catalysis B, Enzymatic, 129, pp.29-36; doi:10.1016/j.molcatb.2016.04.004.


77. Dimarogona M., Topakas* E. (2015) Chapter: Regulation and heterologous expression of lignocellulosic enzymes in Aspergillus. Book Chapter in “New and future developments in microbial biotechnology and bioengineering: Aspergillus system properties and applications”. Gupta V.K., Elsevier , in Press.


76. Katsimpouras C., Antonopoulou I., Christakopoulos P., Topakas* E. (2015). Chapter: Role and applications of feruloyl esterases in biomass bioconversion. Book Chapter, in “Microbial Enzymes in Bioconversions of Biomass”. Gupta V.K., Springer, doi: 10.1007/978-3-319-43679-1_5.


75. Xiros C., Topakas E., Christakopoulos P. (2016). Chapter 2: Hydrolysis and fermentation for cellulosic ethanol production, in Advances in Bioenergy: The Sustainability Challenge. Eds Lund P.D., Byrne J., Berndes G., Vasalos L.A., Chichester, UK, John Wiley & Sons Ltd.


74. Karnaouri A., Matsakas L., Topakas E., Rova U., Christakopoulos P. (2016). Development of thermophilic tailor-made enzyme mixtures for the bioconversion of agricultural and forest residues. Frontiers in Microbiology,


73. Zerva A., Zervakis G.I., Christakopoulos P., Topakas* E. (2016). Degradation of olive mill wastewater by the induced extracellular ligninolytic enzymes of two wood-rot fungi. Journal of Environmental Management, 203, pp. 791-798;


72. Gupta* Udatha D.B.R.K., Topakas* E., Salazar M., Olsson L., Anderssen M.R., Panagiotou G. (2015). Deciphering the signaling mechanisms of the plant cell wall degradation machinery in Aspergillus oryzae. BMC Systems Biology, 9:77; DOI 10.1186/s12918-015-0224-5 (*equal contribution)


71. Sunner H., Charavgi M-D., Olsson L., Topakas E., Christakopoulos P. (2015). Glucuronoyl esterase screening and characterization assays utilizing commercially available benzyl glucuronic acid ester. Molecules, 20, pp. 17807-17817; doi:10.3390/molecules201017807.


70. Kanelli M., Vasilakos S., Nikolaivits E., Ladas S., Christakopoulos P. Topakas* E. (2015) Surface modification of poly(ethylene terephthalate)(PET) fibers by a cutinase from Fusarium oxysporum. Process Biochemistry, 50, pp. 1885-1892.


69. Dimarogona M., Nikolaivits E., Kanelli M., Christakopoulos P., Sandgren M., Topakas* E. (2015). Structural and functional studies of a Fusarium oxysporum cutinase with polyethylene terephthalate modification potential. Biochimica et biophysica Acta, 1850, pp. 2308-2317.


68. Zeleke J., Nikodinovic-Runic J., Topakas* E. (2015). Naturally decaying agricultural residues: Enriched resources for mining novel lignocellulolytic enzymes. Journal of Proteomics and Enzymology, 4:1 doi:10.4172/jpe.1000120.


67. Zerva A., Christakopoulos P., Topakas* E. (2015). Characterization and application of a novel class II thermophilic peroxidase from Myceliophthora thermophila in biosynthesis of polycatechol. Enzyme and Microbial Technology, 75-76, pp. 49-56.


66. Sfakianakis P., Topakas E., Tzia C. (2015). Comparative study on high intensity ultrasound and pressure milk homogenization: effect on the kinetics of yogurt fermentation process. Food and Bioprocess Technology, 8(3), pp. 548-557.


65. Karnaouri* A., Topakas* E., Antonopoulou I., Christakopoulos P. (2014). Genomic insights into the fungal lignocellulolytic system of Myceliophthora thermophila. Frontiers in Microbiology, doi: 10.3389/fmicb.2014.00281. (*equal contribution)


64. Kanelli M., Douka A., Vouyiouka S., Papaspyrides C.D., Topakas E., Papaspyridi L.-M., Christakopoulos P. (2014). Production of biodegradable polyesters via enzymatic polymerization and solid state finishing. Journal of Applied Polymer Science, 131(19), pp 1-8.


63. Katsimpouras C., Christakopoulos P., Topakas* E. (2014). Chapter 11. Fermentation and enzymes. Book Chapter, in “Food Engineering Handbook, Food Process Engineering”. Varzakas T., Tzia C., CRC- Press, Wiley Blackwell, pp 489-518. (*Corresponding author)


62. Topakas E., Christakopoulos P. (2014). Screening and purification of recombinant lignocellulolytic enzymes. Book Chapter, in “Methods in Molecular Biology, Protein Downstream processing: Methods and Protocols”. Ν. Labrou (Ed), Humana Press-Springer, Volume 1129, pp 517-526.


61. Topakas E. (2014). Biofuels from microalgae. Book Chapter, in “Biofuels, sustainable energy.” Karnavos N., Lappas A., Marnellos G. (Eds.), TEE.


60. Matsakas L., Topakas E., Christakopoulos P. (2014) New trends in microbial production of 3-hydroxypropionic acid. Current Biochemical Engineering, 1, pp. 141-154.


59. Katsimpouras C., Benarouche A., Navarro D., Karpusas M., Dimarogona M., Berrin J.-G., Christakopoulos P., Topakas* E. (2014) Enzymatic synthesis of model substrates recognized by glucuronoyl esterases from Podospora anserina and Myceliophthora thermophila. Applied and Microbial Biotechnology, 98(12), pp.5507-5516.


58. Karnaouri* A.C., Topakas* E., Christakopoulos P. (2014). Cloning, expression and characterization of a thermostable GH7 endoglucanase from Myceliophthora thermophila capable of high-consistency enzymatic liquefaction. Applied Microbiology and Biotechnology, 98(1), pp. 231-242. (*equal contribution)


57. Dimarogona M., Topakas E., Christakopoulos P. (2013). Recalcitrant polysaccharide degradation by novel oxidative biocatalysts. Applied Microbiology and Biotechnology, 97, pp. 8455-8465.


56. Topakas E., Panagiotou G., Christakopoulos P. (2013). 9. Xylanases: Characteristics, sources, production and applications. Book Chapter, in “Bioprocessing Technologies in Integrated Biorefinery for Production of Biofuels, Biochemicals, and Biopolymers from Biomass.” Yang, ST, El-Ensashy, H, Thongchul, N (Eds.), John Wiley & Sons, Inc), DOI: 10.1002/9781118642047.ch9. Publication date: July 12, 2013


55. Thörn C., Udatha D.B.R.K.U., Zhou H.,Christakopoulos P., Topakas E., Olsson L. (2013). Understanding the pH dependent immobilization efficacy of feruloyl esterase-C on mesoporous silica and its structure-activity changes. Journal of Molecular Catalysis B: Enzymatic, 93, pp. 65-72.


54. Karnaouri A.C., Topakas* E., Paschos T., Taouki I., Christakopoulos P. (2013). Cloning, expression and characterization of an ethanol tolerant GH3 β-glucosidase from Myceliophthora thermophila. PeerJ, 1, e46 (*Corresponding author)


53. Charavgi M., Dimarogona M., Topakas E., Christakopoulos P., Chrysina E. (2013). The structure of a novel glucuronoyl esterase from Myceliophthora thermophila gives new insights on its role as a potential biocatalysts. Acta Crystallographica Section D, 69(1), pp. 63-73.


52. Vouyiouka S.N., Topakas E., Katsini A., Papaspyrides C.D., Christakopoulos P. (2013). A green route for the preparation of aliphatic polyesters via lipase-catalyzed prepolymerization and low-temperature post polymerization. Macromolecular Materials and Engineering, 298, pp. 679-689.


51. Christakopoulos P., Topakas E. (2012). Editorial Note: Advances in Enzymology and Enzyme Engineering. Computational and Structural Biotechnology Journal, 2(3),


50. Dimarogona M., Topakas E., Christakopoulos P. (2012). Cellulose degradation by oxidative enzymes. Computational and Structural Biotechnology Journal, 2(3),


49. Xiros* C., Topakas* E., Christakopoulos P. (2012). Hydrolysis and fermentation for cellulosic ethanol production. Wiley Interdisciplinary Review: Energy and Environment, doi: 10.1002/wene.49. (*equal contribution)


48. Papaspyridi L.-M., Aligiannis N., Topakas E., Christakopoulos P., Skaltsounis A.-L., Fokialakis N. (2012). Submerged fermentation of the edible mushroom Pleurotus ostreatus in a batch stirred tank bioreactor as a promising alternative for the effective production of bioactive metabolites. Molecules, 17(3), pp. 2714-2724.


47. Dimarogona M., Topakas E., Olsson L., Christakopoulos P. (2012). Lignin boosts the cellulase performance of a GH-61 enzyme from Sporotrichum thermophile. Bioresource Technology, 110, pp. 480-487.


46. Dimarogona M., Topakas E., Christakopoulos P., Chrysina E.D. (2012). The structure of a GH10 xylanase from Fusarium oxysporum reveals the presence of an extended loop on top of the catalytic cleft. Acta Crystallographica Section D, 68(7), pp. 735-742.


45. Xiros C., Vafiadi C., Topakas E., Christakopoulos P. (2012). Decrement of cellulose recalcitrance by treatment with ionic liquid (1-ethyl-3-methylimidazolium acetate) as a strategy to enhance enzymatic hydrolysis. Journal of Chemical Technology & Biotechnology, 87, pp. 629-634.


44. Topakas* E., Moukouli* M., Dimarogona M., Christakopoulos P. (2012). Expression, characterization and structural modelling of a feruloyl esterase from the thermophilic fungus Myceliophthora thermophila. Applied Microbiology and Biotechnology, 94(2), pp. 399-411. (*equal contribution)


43. Panagiotou* G., Topakas* E., Olivarres R., Christakopoulos P. & Olsson L. (2011). Feruloyl esterases as biosynthetic tools for the organic synthesis of nutraceutical compounds. Book Chapter, in ‘Encyclopedia of Biotechnology in Agriculture and Food.’ Heldman D.R., Brigdes A., Hoover D. & Wheeler M.B. (Eds.), Taylor & Francis, pp. 448-453. Publication date: August 24, 2011. (equal contribution)


42. Panagiotou* G., Topakas* E., Moukouli M., Christakopoulos P., Olsson L. (2011). Studying the ability of Fusarium oxysporum and recombinant Saccharomyces cerevisiae to efficiently cooperate in decomposition and ethanolic fermentation of wheat straw. Biomass and Bioenergy, 35, pp. 3727-3732. (*equal contribution)


41. Moukouli M., Topakas E., Christakopoulos P. (2011). Cloning and optimized expression of a GH-11 xylanase from Fusarium oxysporum in Pichia pastoris. New Biotechnology, 28, pp. 369-374.


40. Kourtoglou E., Anasotzis G., Mamma D., Topakas E., Hatzinikolaou D., Christakopoulos P. (2011). Constitutive expression, purification and characterization of a phosphoglucomutase from Fusarium oxysporum. Enzyme and Microbial Technology, 48, pp. 217-224.


39. Topakas E., Moukouli M., Dimarogona M., Vafiadi C., Christakopoulos P. (2010). Functional expression of a thermophilic glucuronoyl esterase from Sporotrichum thermophile: identification of the nucleophilic serine. Applied Microbiology and Biotechnology, 87, pp. 1765-1772.


38. Topakas E.*, Kyriakopoulos S., Biely P., Hirsch J., Vafiadi C. & Christakopoulos P. (2010). Carbohydrate esterases of family 2 are 6-O-deacetylases. FEBS Letters, 584, pp. 543-548. (*Corresponding author)


37. Vafiadi C., Christakopoulos P., Topakas E.* (2009). Purification, characterization and mass spectrometric identification of two thermophilic xylanases from Sporotrichum thermophile. ProcessBiochemistry, 45, pp. 419-424. (*Corresponding author)


36. Mamma D., Topakas E., Vafiadi C. & Christakopoulos P. (2009). 14. Biotechnological potential of fruit processing industry residues. Book Chapter, in ‘Biotechnological potential of agro-industrial residues for bioprocesses.’ Nigam P.S. & Pandey A. (Eds.), Springer, Part III, pp. 273-291. ISBN: 978-1-4020-9941-0


35. Xiros C., Moukouli M., Topakas E. & Christakopoulos P. (2009). Factors affecting ferulic acid release from brewer’s spent grain by Fusarium oxysporum enzymatic system. Bioresource Technology, 100, pp. 5917-5921.


34. Vafiadi C., Topakas E., Biely P. & Christakopoulos P. (2009). Purification, characterization and mass spectrometric sequencing of a thermophilic glucuronoyl esterase from Sporotrichum thermophile. FEMS Microbiology Letters, 296, pp. 178-184.


33. Montanier C., Money V.A, Pires V.M.R., Flint J.E., Pinheiro B.A., Goyal A., Prates J.A.M., Izumi A., Stalbrand H., Morland C., Cartmell A., Kolenova K., Topakas E., Dodson E.J., Bolam D.N., Davies G.J., Fontes C.M.G.A. & Gilbert H.J. (2009). The active site of a carbohydrate esterase displays divergent catalytic and non-catalytic binding functions. PLOS Biology, 7 (3), e1000071, (together with  “Gene sharing yields an enzyme with two binding sites in one subunit” (CaitlinSedwick, PLOSBiology, 7(3),e1000064.)


32. Emami* K., Topakas* E., Nagy* T., Henshaw J., Nelson K.E., Jackson K.A., Mongodin E.F., Murray J.W., Gilbert H.J. & Lewis R.J. (2009). Regulation of the xylan degrading apparatus of Cellvibrio japonicus by a novel two component system. Journal of Biological Chemistry, 284, pp. 1086-1096. (*equal contribution)


31. Vafiadi C., Topakas E., Nahmias V.R., Faulds C.B.& Christakopoulos P. (2009). Feruloyl esterase-catalysed synthesis of glycerol sinapate using ionic liquids mixtures. Journal of Biotechnology, 139, pp. 124-129.


30. Cartmell* A., Topakas* E., Ducros* V.M.-A., Suits M.D.L, Davies G.J. & Gilbert H.J. (2008). The Cellvibrio japonicus mannanase CjMan26C displays a unique exo-mode of action that is conferred by subtle changes to the distal region of the active site. Journal of Biological Chemistry, 283, pp. 34403-34413. (*equal contribution)


29. Panagiotou G.,Papadakis M.A.,Topakas E., Olsson L. & Christakopoulos P. (2008). Identification of NADH kinase activity in filamentous fungi and structural modelling of the novel enzyme from Fusarium oxysporum. Process Biochemistry, 43, pp. 1114-1120.


28. Kourtoglou E., Mamma D., Topakas E. & Christakopoulos P. (2008). Purification, characterization and mass spectrometric sequencing of transaldolase from Fusarium oxysporum. Process Biochemistry,43, pp. 1094-1101.


27. Moukouli* M., Topakas* E. & Christakopoulos P. (2008). Cloning, characterization and functional expression of an alkalitolerant type C feruloyl esterase from Fusarium oxysporum. Applied Microbiology and Biotechnology, 79, pp. 245-254. (*equal contribution)


26. Xiros C., Topakas E., Katapodis P., & Christakopoulos P. (2008). Evaluation of Fusarium oxysporum as an enzyme factory for the hydrolysis of brewer’s spent grain with improved biodegradability for ethanol production. Industrial Crops and Products, 28, pp. 213-224.


25. Vafiadi C., Topakas E. & Christakopoulos P. (2008). Preparation of multipurpose cross-linked enzyme aggregates and their application to production of alkyl ferulates. Journal of Molecular Catalysis B: Enzymatic, 54, pp. 35-41.


24. Xiros C., Topakas E., Katapodis P. & Christakopoulos P. (2008). Hydrolysis and fermentation of brewer’s spent grain by Neurospora crassa. Bioresource Technology, 99, pp. 5427-5435.


23. Vafiadi C., Topakas E., Alissandratos A., Faulds C.B. & Christakopoulos P. (2008). Enzymatic synthesis of butyl hydroxycinnamates and their inhibitory effects on LDL-oxidation. Journal of Biotechnology, 133, pp. 497-504.


22. Topakas E. & Christakopoulos P. (2007). Microbial xylanolytic carbohydrate esterases. Book Chapter 6, in ‘Industrial Enzymes: Structure, Function and Applications.’ Polaina J. & MacCabe A.P. (Eds.), Springer, ISBN: 978-1-4020-5376-4.


21. Vafiadi C., Topakas E., Alderwick L.J., Besra G.S. & Christakopoulos P. (2007). Chemoenzymatic synthesis of feruloyl D-arabinose as potential anti-mycobacterial agent. Biotechnology Letters, 29, pp.1771-1774.


20. Vafiadi C., Topakas E., Bakx E.J., Schols H.A. & Christakopoulos P. (2007). Structural characterization by ESI-MS of feruloylated arabino-oligosaccharides synthesised by chemoenzymatic esterification. Molecules, 12, pp. 1367-1375.


19. Topakas E., Vafiadi C. & Christakopoulos P. (2007). Microbial production, characterization and applications of feruloyl esterases. Process Biochemistry, 42, pp. 497-509.


18. Puchart V., Vršanská M., Mastihubová M., Topakas E., Vafiadi C., Faulds C.B., Tenkanen M., Christakopoulos P. & Biely P. (2007). Substrate and positional specificity of feruloyl esterases for monoferuloylated and monoacetylated 4-nitrophenyl glycosides. Journal of Biotechnology, 127, pp. 235-243.


17. Vafiadi C., Topakas E.& Christakopoulos P. (2006). Regioselective esterase-catalyzed feruloylation of L-arabinobiose. Carbohydrate Research, 341, pp. 1992-1997.


16. Vafiadi C., Topakas E., Christakopoulos P. & Faulds C.B. (2006). The feruloyl esterase system of Talaromyces stipitatus: determining the hydrolytic and synthetic specificity of TsFaeC. Journal of Biotechnology, 125, pp. 210-221.


15. Vafiadi C., Topakas E., Wong K.K.Y., Suckling I.D. & Christakopoulos P. (2005). Mapping the hydrolytic and synthetic selectivity of a type C feruloyl esterase (StFaeC) from Sporotrichum thermophile using alkyl ferulates. Tetrahedron: Asymmetry (Invited paper), 16, pp. 373-379.


14. Topakas E., Vafiadi C., Stamatis H. & Christakopoulos P. (2005). Sporotrichum thermophile type C feruloyl esterase (StFaeC): Purification, characterization, and its use for phenolic acid (sugar) ester synthesis. Enzyme and Microbial Technology, 36, pp. 729-736.


13. Topakas E., Christakopoulos P. & Faulds C.B. (2005). Comparison of mesophilic and thermophilic feruloyl esterases: characterization of their substrate specificity for methyl phenylalkanoates. Journal of Biotechnology, 115, pp. 355-366.


12. Topakas E., Christakopoulos P. (2004). Production and partial characterization of alkaline feruloyl esterases by Fusarium oxysporum during submerged batch cultivation. World Journal of Microbiology and Biotechnology, 20, pp. 245-250.


11. Topakas E., Stamatis H., Biely P., Christakopoulos P. (2004). Purification and characterization of a type B feruloyl esterase (StFAE-A) from the thermophilic fungus Sporotrichum thermophile. Applied Microbiology and Biotechnology, 63, pp.686-690.


10. Vardakou M., Katapodis P., Topakas E., Kekos D., Macris B.J., Christakopoulos P. (2004). Synergy between enzymes involved in degradation of insoluble wheat flour arabinoxylan. Innovative Food Science and Emerging Technologies, 5, pp. 107-112.


9. Gkargkas K., Mamma D., Nedev G., Topakas E., Christakopoulos P., Kekos D., Macris B.J. (2004). Studies on a N-acetyl-β-D-glycosaminidase produced by Fusarium oxysporum F3 grown in solid-state fermentation. Process Biochemistry, 39 (11), pp. 1599-1605.


8. Topakas E., Kalogeris E., Kekos D., Macris B.J., Christakopoulos P. (2004). Production of phenolics from corn cobs by coupling enzymic treatment and solid state fermentation. Engineering in Life Sciences, 4 (3), pp. 283-286.


7. Topakas E., Stamatis H., Mastihubova, M., Biely P., Kekos D., Macris B.J., Christakopoulos P. (2003) Purification and characterization  of a Fusarium oxysporum feruloyl esterase (FoFAE-I) catalysing transesterification of phenolic acid esters. Enzyme and Microbial Technology, 33, pp. 729-737.


6. Topakas E., Stamatis H., Biely P., Kekos D., Macris B.J., Christakopoulos P. (2003). Purification and characterization of a feruloyl esterase from Fusarium oxysporum catalyzing esterification of phenolic acids in ternary water-organic solvent mixtures. Journal of Biotechnology, 102 (1), pp. 33-44.


5. Topakas E., Kalogeris E., Kekos D., Macris B.J., Christakopoulos P. (2003).Production and partial characterization of feruloyl esterase by Sporotrichum thermophile under solid-state fermentation. Process Biochemistry, 38, pp. 1539-1543.


4. Panagiotou G., Topakas E., Economou L., Kekos D., Macris B.J., Christakopoulos P. (2003). Induction, purification, and characterization of two extracellular a-L-arabinofuranosidases from Fusarium oxysporum. Canadian Journal Microbiology, 49, pp. 639-644.


3. Topakas E., Kalogeris E., Kekos D., Macris B.J., Christakopoulos P. (2003). Bioconversion of ferulic acid into vanillic acid by the thermophylic fungus Sporotrichum thermophile. Lebensmittel-Wissenschaft und - Technologie, 36 (6), pp. 561-565.


2. Topakas E., Katapodis P., Kekos D., Macris B.J., Christakopoulos P. (2003). Production and partial characterization of xylanase by Sporotrichum thermophile under solid-state fermentation. World Journal of Microbiology and Biotechnology, 19 (2), pp. 195-198.


1. Kalogeris E., Iniotaki F., Topakas E., Christakopoulos P., Kekos D., Macris B.J. (2003). Performance of an intermittent agitation rotating drum type bioreactor for solid-state fermentation of wheat straw. BioresourceTechnology, 86 (3), pp. 207-213.

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