Efstratios Nikolaivits

 

Education

 

06/2015 – 12/2019

Ph.D. in Industrial Biotechnology

National Technical University of Athens (NTUA)

School of Chemical Engineering

Biotechnology Laboratory

Ph.D. Thesis: Discovery of novel fungal biocatalysts and application in bioremediation of chlorinated aromatic pollutants

 

10/2007 – 3/2014

Diploma in Chemical Engineering

National Technical University of Athens (NTUA)

School of Chemical Engineering

Upper Second-Class Honours (7.24/10)

Diploma Thesis: Engineering of the heterologous expression and characterization of a cutinase from Fusarium oxysporum

 

 

Languages

 

Greek (Native speaker)

English (C2 Level)

German (B2 Level)

 

 

Awards

 

2021

Best Poster Award (2nd Prize) at the 8th International Conference on Sustainable Solid Waste Management

 

2020

"Iakovos Giourounlian Award-BIOHALCO Foundation” for best industrially relevant 2019 Chemical Engineering PhD Thesis (2nd Prize)

 

2020

Dimitris N. Chorafas Foundation Prize for best 2019 NTUA PhD Thesis (1st Prize)

 

12/2016 – 12/2019

PhD thesis Scholarship from the Greek State Scholarship Foundation

 

02/2017 – 03/2017

COST Action CM1303 ("Systems Biocatalysis") fellowship for short-term scientific mission to the TECHNION-Israel Institute of Technology

 

 

Work Experience

 

 

01/2020 - 09/2021

Post-Doc Researcher

National Technical University of Athens (NTUA)

“Bio Innovation of a Circular Economy for Plastics – BioICEP”, Horizon 2020

 

01/2021-08/2021

Post-Doc Researcher

CERTH-CPERI

“Novel Conversion Technologies of Waste Biomass to Food additives and Fine Chemicals – NoWasteBiotech”, GSRT/HFRI funded

 

04/2020 - 12/2020

Post-Doc Researcher

CERTH-iBO

Holistic approach along the production cycle of Stevia Rebaudiana plant cultivated in Greece, via combined application of innovative methods of Precision Agriculture and bitter aftertaste removal techniques – TasteSTEVIA”, EU-ERDF/Greek funds “RESEARCH – CREATE – INNOVATE”

 

11/2015 – 09/2019

Research Assistant

Nat. Kapodistrian University of Athens

Tools And Strategies to access original bioactive compounds by Cultivating MARine invertebrates and associated symbionts – TASCMAR”, Horizon 2020

 

02/2017 – 03/2017

Research Assistant

TECHNION-Israel Institure of Technology

"Bioconversion of cinnamic acid derivatives towards the production of caffeic acid using engineered monooxygenase and PPO", EU COST Action CM1303 "Systems Biocatalysis"

 

10/2014 – 09/2015

Research Assistant

National Technical University of Athens (NTUA)

The Sustainable Integrated Method for the Production of Lignocellulosic Ethanol”, SYNERGASIA 2011

 

9/2011 –12/2011

Intern

National Hellenic Research Foundation

Institute of Biology, Medicinal Chemistry and Biotechnology – Industrial Enzymology Unit

 

 

Teaching and Supervision Experience

 

06/2015 – 12/2019

Teaching assistant at the laboratory courses: Enzyme and Microbial Technology and Principles of Biotechnology

Biotechnology Laboratory, School of Chemical Engineering, NTUA

 

2015 – present

Thesis Supervision, Biotechnology Laboratory, School of Chemical Engineering, NTUA

 Undergrad students: K. Makryniotis (2021), P. Dimopoulou (2020), E. Prinea (2020), R. Siaperas (2019), M. Panagiotopoulou (2018), A. Agrafiotis (2017), A. Chalima (2016-Award), I. Karagiannaki (2016), G. Makris (2015), G.F. Norra (2015)

Grad students: G. Taxeidis (2020-), R. Siaperas (2020-)

 

 

Reviewer at peer-reviewed Journals

 

Journal of Functional Biomaterials – MDPI Publishing

Applied Sciences – MDPI Publishing (IF 2.679)

Royal Society Open Science – Royal Society Publishing (IF 2.649)

Water – MDPI Publishing (IF 3.103)

World Journal of Microbiology and Biotechnology – Springer (IF 3.312)

International Journal of Environmental Research and Public Health – MDPI Publishing (IF 3.390)

Process Biochemistry – Elsevier (IF 3.757)

Microorganisms – MDPI Publishing (IF 4.128)

Toxics – MDPI Publishing (IF 4.146)

Catalysts (Reviewer Board Member) – MDPI Publishing (IF 4.146)

Foods – MDPI Publishing (IF 4.350)

Molecules – MDPI Publishing (IF 4.411)

Food and Bioproducts Processing – Elsevier (IF 4.481)

Antibiotics (Reviewer Board Member) – MDPI Publishing (IF 4.639)

Biomolecules – MDPI Publishing (IF 4.879)

Food & Function – Royal Society of Chemistry (IF 5.396)

Journal of Fungi – MDPI Publishing (IF 5.816)

International Journal of Molecular Sciences – MDPI Publishing (IF 5.923)

Computational and Structural Biotechnology Journal – Elsevier (IF 7.271)

 

 

Peer-reviewed Publications

 

19. Nikolaivits, E.; Pantelic, B., Azeem, M., Taxeidis, G., Babu, R., Topakas, E., Brennan Fournet, M., Nikodinovic-Runic, J. Progressing plastics circularity: A review of mechano-biocatalytic approaches for waste plastic (re)valorization. Front. Bioeng. Biotech. 2021, 9, 535

18. Nikolaivits, E.; Valmas, A.; Dedes, G.; Topakas, E.; Dimarogona, M. Considerations on activity determinants of fungal polyphenol oxidases based on mutational and structural studies. Appl. Environ. Microbiol. 2021, AEM.00396-21

17. Djapovic M.; Milivojevic D.; Ilic-Tomic T.; Lješević M.; Nikolaivits E.; Topakas E.; Maslak V.; Nikodinovic-Runic J. Synthesis and characterization of polyethylene terephthalate (PET) precursors and potential degradation products: Toxicity study and application in discovery of novel PETases. Chemosphere. 2021, Accepted

16. Nikolaivits, E.; Siaperas R.; Agrafiotis, A.; Ouazzani J.; Gioti A.; Topakas, E. Functional and transcriptomic investigation of laccase activity in the presence of PCB29 identifies two novel enzymes and the multicopper oxidase repertoire of a marine-derived fungus. Sci. Total Environ. 2021, In Press

15. Nikolaivits, E.; Agrafiotis, A.; Baira, E.; Le Goff, G.; Tsafantakis, N.; Chavanich, S.; Benayahu, Y.; Ouazzani, J.; Fokialakis, N.; Topakas, E. Degradation mechanism of 2,4-dichlorophenol by fungi isolated from marine invertebrates. Int. J. Mol. Sci. 2020, 21(9), 3317

14. Gioti A.; Siaperas R.; Nikolaivits E.; Le Goff G.; Ouazzani J.; Kotoulas G.; Topakas E. Draft genome sequence of a Cladosporium species isolated from the mesophotic ascidian Didemnum maculosum. Microbiol. Resour. Announc., 2020, 9, e00311-20.

13. Paz A.; Nikolaivits E.; Topakas E. Valorization of olive mill wastewater towards the production of L-asparaginases. Biomass Convers. Biorefin. 2020, 1-8.

12. Nikolaivits, E.; Agrafiotis, A.; Termentzi, A.; Machera, K.; Le Goff, G.; Álvarez, P.; Chavanich, S.; Benayahu, Y.; Ouazzani, J.; Fokialakis, N.; Topakas, E. Unraveling the detoxification mechanism of 2,4-dichlorophenol by marine-derived mesophotic symbiotic fungi isolated from marine invertebrates. Mar. Drugs. 2019, 17, 564.

11. Mandic, M.; Djokic, L.; Nikolaivits, E.; Prodanovic, R.; O’Connor, K.; Jeremic, S.; Topakas, E.; Nikodinovic-Runic, J. Identification and characterization of new laccase biocatalysts from Pseudomonas species suitable for degradation of synthetic textile dyes. Catalysts. 2019, 9, 629.

10. Nikolaivits, E.; Kanelli, M.; Dimarogona, M.; Topakas E. A middle-aged enzyme still in its prime: Recent advances in the field of cutinases. Catalysts. 2018, 8(12), 612.

9. Nikolaivits, E.; Dimarogona, M.; Karagiannaki I.; Chalima A.; Fishman A.; Topakas E. Characterization and protein engineering of a novel versatile fungal polyphenol oxidase with chlorophenol bioremediation potential. Appl. Environ. Microbiol. 2018, In Press

8. Nikolaivits, E.; Termentzi, A.; Skaltsounis, A.-L.; Fokialakis, N.; Topakas, E. Enzymatic tailoring of oleuropein from Olea europaea leaves and product identification by HRMS/MS spectrometry. J. Biotechnol. 2017, 253, 48–54.

7. Nikolaivits, E.; Makris, G.; Topakas, E. Immobilization of a cutinase from Fusarium oxysporum and application in pineapple flavor synthesis. J. Agric. Food Chem. 2017, 65 (17), 3505–3511.

6. Nikolaivits, E.; Dimarogona, M.; Fokialakis, N.; Topakas, E. Marine-derived biocatalysts: Importance, accessing, and application in aromatic pollutant bioremediation. Front. Microbiol. 2017, 8, 265.

5. El-Gamal, R.; Nikolaivits, E.; Zervakis, G. I.; Abdel-Maksoud, G.; Topakas, E.; Christakopoulos, P. The use of chitosan in protecting wooden artifacts from damage by mold fungi. Electron. J. Biotechnol. 2016, 24, 70–78.

4. Nikolaivits, E.; Kokkinou, A.; Karpusas, M.; Topakas, E. Microbial host selection and periplasmic folding in Escherichia coli affect the biochemical characteristics of a cutinase from Fusarium oxysporum. Protein Expr. Purif. 2016, 127, 1–7.

3. Nikolaivits, E.; Norra, G.-F.; Voutsas, E.; Topakas, E. Cutinase from Fusarium oxysporum catalyzes the acylation of tyrosol in an aqueous medium: optimization and thermodynamic study of the reaction. J. Mol. Catal. B Enzym. 2016, 129, 29–36.

2. Kanelli, M.; Vasilakos, S.; Nikolaivits, E.; Ladas, S.; Christakopoulos, P.; Topakas, E. Surface modification of poly(ethylene terephthalate) (PET) fibers by a cutinase from Fusarium oxysporum. Process Biochem. 2015, 50 (11), 1885–1892.

1. Dimarogona, M.; Nikolaivits, E.; Kanelli, M.; Christakopoulos, P.; Sandgren, M.; Topakas, E. Structural and functional studies of a Fusarium oxysporum cutinase with polyethylene terephthalate modification potential. Biochim. Biophys. Acta - Gen. Subj. 2015, 1850 (11), 2308–2317.

 

 

Conference Publications and Non-Peer reviewed Publications

 

6. Nikolaivits, E., Dimopoulou, P., Maslak, V., Nikodinovic-Runic, J., Topakas, E. Discovery and biochemical characterization of a novel polyesterase for the degradation of synthetic plastics. Chem. Proc. 2020, 2, 33.

5. Nikolaivits, E.; Dimarogona M.; Karagiannaki I.; Chalima A.; Fishman A.; Topakas, E. Discovery and mutation of a novel enzyme for the removal of environmental pollutants. Atlas of Science 2020, May 20

4. Nikolaivits, E.; Agrafiotis, A.; Fokialakis, N.; Topakas, E. Biodegradation of 2, 4, 5-trichlorobiphenyl (PCB29) by marine-derived mesophotic fungi. New Biotechnol. 2018, 44, S136–S137.

3. Nikolaivits, E.; Topakas, E. Improved characteristics of an industrial biocatalyst expressed in engineered E. coli. Atlas of Science 2017, February 8

2. Nikolaivits, E.; Termentzi, A.; Skaltsounis, A.; Fokialakis, N.; Topakas, E. Enzymatic tailoring of oleuropein isolated from Olea europaea leaves. Planta Med. 2016, 81 (S 01), S1–S381.

1. Topakas, E.; Nikolaivits, E.; Kanelli, M.; Christakopoulos, P. A cutinase from Fusarium oxysporum with potential for PET surface modification. N. Biotechnol. 2014, 31, Supple (0), S90.

 

 

Scientific workshops and training schools

 

4. “Webinars on Fridays” organized by Hellenic National Initiative “MikroBioKosmos” - Oral presentation

“Plastic Biodegradation: Can the existing microbial enzymatic system be a sustainable solution?”

3. 3rd International Meeting of the Hellenic Crystallographic Association’s Young Researchers (Patras, Greece 2019) - Oral presentation,

“Structural and functional characterization of a novel fungal polyphenol oxidase and its variants”

2. Diffraction Data Collection Using Synchrotron Radiation  Workshop by the German Crystallographic Society – DGK-AK1 (Berlin, Germany 2019) - Oral presentation,

“Structural studies of a novel GH30 xylanase with unique catalytic properties“

1. COST Action “Systems Biocatalysis” Training School (Siena, Italy 2016) - Oral presentation,

“Heterologous expression, crystal structure, evolution and immobilization of a cutinase with a variety of applications“

 

 

International Conferences

 

 

16. 8th International Conference on Sustainable Solid Waste Management (Virtual, 2021)

“Discovery of a novel PETase-like enzyme for the degradation of plastic waste” – 2nd Best Poster Award

15. European Federation of Biotechnology Conference (Virtual, 2021)

“The ability of a known plant-biomass degrading fungus to break down polyurethane” and

“Selective degradation of polyethylene terephthalate (PET)-related substrates by Penicillium sp. MM41”

14. 15th BIOTRANS (Virtual, 2021)

“Repurposing of plant-biomass hydrolyzing enzymes for the degradation of polyethylene terephthalate (PET)”,

“Investigation of the dual function of a GH30_7 xylanase through structural studies” and

“Towards novel polyethylene terephthalate (PET)-ases: Whole-cell biotransformation of PET-related substrates”

13. Protein Data Bank Symposium-PDB50 (Virtual, 2021)

“Structural and functional characterization of a novel fungal GH30_7 xylanase with xylobiohydrolase auxiliary activity”

12. 1st International Electronic Conference on Catalysis Sciences (Online, 2020)

“Discovery and biochemical characterization of a novel polyesterase for the degradation of synthetic plastics” – Best Paper Award

11.14th BIOTRANS (Groningen, the Netherlands 2017)

“Structural and functional characterization of a novel fungal GH30_7 xylanase with xylobiohydrolase auxiliary activity” and

“Discovery of novel enzymes from marine-derived mesophotic fungi able to bioconvert chlorinated aromatic pollutants”

10. 6th International Conference on Novel Enzymes (Darmstadt, Germany 2018)

“Discovery of a novel enzymatic activity from a marine-derived fungus: Catalase with catechol dioxygenase activity“

“Expression and purification of a novel laccase from a marine-derived mesophotic PCB-degrading fungus”

9. 18th European Congress On Biotechnology (Geneva, Switzerland 2018)

“Biodegradation of 2,4,5-trichlorobiphenyl (PCB29) by marine-derived mesophotic fungi”

8. 6th International Conference on Sustainable Solid Waste Management (Naxos, Greece 2018) -Oral presentation

“Potential of marine-derived mesophotic fungi for the bioremediation of 2,4-dichlorophenol-contaminated wastewaters”

7. 13th BIOTRANS (Budapest, Hungary 2017)

“Discovery of marine-derived mesophotic biocatalysts for the degradation of persistent organic pollutants” and

“Protein engineering of a fungal Polyphenol oxidase and its potential application in bioconversion of POPs”

6. 8th European Meeting on OxiZymes (Wageningen, the Netherlands 2016)

“Protein engineering of a catechol oxidase and its potential application in bioconversion of POPs”

5. 5th International Conference on Novel Enzymes (Groningen, the Netherlands 2016)

“Protein engineering of a polyphenol oxidase towards enhancement of its monophenolase activity”

4. 12th BIOTRANS (Vienna, Austria 2015)

“Study of the synthetic potential of a Fusarium oxysporum cutinase in water-oil biphasic reaction system”

3. 9th Joint Meeting in Medicinal Chemistry (Athens, Greece 2015)

“Enzymatic tailoring of oleuropein isolated from Olea europaea leaves”

2. 11th Carbohydrate Bioengineering Meeting (Espoo, Finland 2015)

“Structural and functional studies of a Fusarium oxysporum cutinase with poly(ethylene terephthalate) modification potential”

1. 16th European Congress on Biotechnology (Edinburgh, Scotland 2014)

“A cutinase from Fusarium oxysporum with potential for PET surface modification”

 

 

National Conferences

 

7. 8th Mikrobiokosmos Conference (Patras, Greece 2019)

“Exploiting marine fungal biodiversity of the mesophotic zone for biodegradation of organic pollutants”

6. 11th Panhellenic Scientific Conference of Chemical Engineering (Thessaloniki, Greece 2017)

“Study of the enzymatic polymerization and mass postpolymerization towards poly(cuccinic butyl ester)”

5. 7th Mikrobiokosmos Conference (Athens, Greece 2017)

“Protein engineering of a polyphenoloxidase from fungus Myceliophthora thermophila aiming to the increase its monophenolase activity”

4. 6th Mikrobiokosmos Conference (Athens, Greece 2017)

“Discovery of marine-derived fungi with potential for persistent organic pollutant degradation”

3. 10th Panhellenic Scientific Conference of Chemical Engineering (Patras, Greece 2015)

“Acylation of tyrosol in biphasic systems using a cutinase from the fungus Fusarium oxysporum”

2. 6th Mikrobiokosmos Conference (Athens, Greece 2015) - Oral presentation

“Structural and functional studies of a cutinase from Fusarium oxysporum with synthetic activity in water-oil biphasic systems and thermodynamic study of the reaction system”

1. 9th Panhellenic Scientific Conference of Chemical Engineering (Athens, Greece 2013)

“Surface modification of synthetic textile products using a cutinase from the fungus Fusarium oxysporum”

 

General Information

 

Location & Contact details

 

Copyright ® 2016. designed by ck & et